La identificación de criminales a través del ADN (página 9)

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D16S539

Other Names	Chromosomal Location	GenBank Accession
D16	16q22-24	G07925; has 11 repeat units AC024591.3

Repeat: [GATA] = GenBank top strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	Promega	GenePrint® PowerPlex™ 1.1
Set 2	CHLC Web Site	5'-GATCCCAAGCTCTTCCTCTT-3' 5'-ACGTTTGTGTGTGCATCTGT-3'
Set 3	ABI	AmpFlSTR® Profiler Plus™

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1	Set 2	Set 3	Repeat Structure	Ref.
5	264 bp	141 bp	233 bp	[GATA]5	721
8	276 bp	145 bp	245 bp	[GATA]8	721
9	280 bp	149 bp	249 bp	[GATA]9	721
10	284 bp	153 bp	253 bp	[GATA]10	721
11	288 bp	157 bp	257 bp	[GATA]11	721
12	292 bp	161 bp	261 bp	[GATA]12	721
13	296 bp	165 bp	265 bp	[GATA]13	721
13.3	299 bp	168 bp	268 bp		*variant allele*
14	300 bp	169 bp	269 bp	[GATA]14	721
15	304 bp	173 bp	273 bp	[GATA]15	721

D18S51

Other Names	Chromosomal Location	GenBank Accession
D18	18q21.3	X91254; has 21 repeats AP001534; has 18 repeats

Repeat: [GAAA] = GenBank top strand

top = 13 repeats GAAA

Reported Primers	Ref.	PCR Primer Sequences
Set 1	7, 369	5'-CAA ACC CGA CTA CCA GCA AC-3' (GAAA strand) 5'-GAG CCA TGT TCA TGC CAC TG-3'
Set 2	ABI	AmpFlSTR® Profiler Plus™
Set 3	Promega	GenePrint® PowerPlex™ 2.1

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1	Set 2	Set 3	3' Flanking Sequence	Repeat Structure	Ref.
7	262 bp	264 bp	286 bp			SGM Plus
8	266 bp	268 bp	290 bp	AAAG AGAGAG	[AGAA]8	716
9	270 bp	272 bp	294 bp	AAAG AGAGAG	[AGAA]9	716
9.2	272 bp	274 bp	296 bp			SGM Plus
10	274 bp	276 bp	298 bp	AAAG AGAGAG	[AGAA]10	716
10.2	276 bp	278 bp	300 bp			SGM Plus
11	278 bp	280 bp	302 bp	AAAG AGAGAG	[AGAA]11	716
12	282 bp	284 bp	306 bp	AAAG AGAGAG	[AGAA]12	716
12.2	284 bp	286 bp	308 bp			SGM Plus
13	286 bp	288 bp	310 bp	AAAG AGAGAG	[AGAA]13	716
13.1	287 bp	289 bp	311 bp			*variant allele*
13.2	288 bp	290 bp	312 bp	AAAG AG AGAGAG	[AGAA]13	369
14	290 bp	292 bp	314 bp	AAAG AGAGAG	[AGAA]14	716
14.2	292 bp	294 bp	316 bp	AAAG AG AGAGAG	[AGAA]14	369
15	294 bp	296 bp	318 bp	AAAG AGAGAG	[AGAA]15	716
15.1	295 bp	297 bp	319 bp			SGM Plus
15.2	296 bp	298 bp	320 bp	AAAG AG AGAGAG	[AGAA]15	369
16	298 bp	300 bp	322 bp	AAAG AGAGAG	[AGAA]16	716
16.2	300 bp	302 bp	324 bp			SGM Plus
16.3	301 bp	303 bp	325 bp			SGM Plus
17	302 bp	304 bp	326 bp	AAAG AGAGAG	[AGAA]17	716
17.1	303 bp	305 bp	327 bp			SGM Plus
17.2	304 bp	306 bp	328 bp	AAAG AGAGAG	[AGAA]17	375
17.3	305 bp	307 bp	329 bp			SGM Plus
18	306 bp	308 bp	330 bp	AAAG AGAGAG	[AGAA]18	716
18.1	307 bp	309 bp	331 bp			SGM Plus
18.2	308 bp	310 bp	332 bp			SGM Plus
19	310 bp	312 bp	334 bp	AAAG AGAGAG	[AGAA]19	716
19.2	312 bp	314 bp	336 bp	AAAG AGAGAG	[AGAA]19	375
20	314 bp	316 bp	338 bp	AAAG AGAGAG	[AGAA]20	716
20.1	315 bp	317 bp	339 bp			SGM Plus
20.2	316 bp	318 bp	340 bp			SGM Plus
21	318 bp	320 bp	342 bp	AAAG AGAGAG	[AGAA]21	716
21.2	320 bp	322 bp	344 bp			SGM Plus
22	322 bp	324 bp	346 bp	AAAG AGAGAG	[AGAA]22	716
22.1	323 bp	325 bp	347 bp			*variant allele*
23	326 bp	328 bp	350 bp	AAAG AGAGAG	[AGAA]23	716
23.1	327 bp	329 bp	351 bp			SGM Plus
24	330 bp	332 bp	354 bp	AAAG AGAGAG	[AGAA]24	716
25	334 bp	336 bp	358 bp	AAAG AGAGAG	[AGAA]25	716
26	338 bp	340 bp	362 bp	AAAG AGAGAG	[AGAA]26	716
27	342 bp	344 bp	366 bp	AAAG AGAGAG	[AGAA]27	716

Other Names

Chromosomal Location

GenBank Accession

21q21.1

M84567; has 26 repeat units

AP000433; has 29.1 repeat units

Repeat: [TCTA], [TCTG]= GenBank top strand

Reported Primers	Ref.	PCR Primer Sequences
Set 1	613, 34	5'-GTG AGT CAA TTC CCC AAG-3' 5'-GTT GTA TTA GTC AAT GTT CTC C-3'
Set 2	5	5'-ATA TGT GAG TCA ATT CCC CAA G-3' 5'-TGT ATT AGT CAA TGT TCT CCA G-3'
Set 3	ABI	AmpFlSTR® Profiler Plus™
Set 4	Promega	GenePrint® PowerPlex™ 2.1

PCR Product Sizes of Observed Alleles

Allele (Repeat #) (Ref. # 34)	Allele (Repeat #) (Ref. # 5)	Set 1,2	Set 3	Set 4	Repeat Structure	Ref.
24	53	202 bp	186 bp	203 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCATA [TCTA]6	716
24.2	54	204 bp	188 bp	205 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8	312
25	55	206 bp	190 bp	207 bp	[TCTA]4 [TCTG]3 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10	312
25.2	56	208 bp	192 bp	209 bp	[TCTA]5 [TCTG]6 [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10	716
26	57	210 bp	194 bp	211 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8	34
26.1		211 bp	195 bp	212 bp		SGM Plus
26.2	58	212 bp	196 bp	213 bp		*variant allele*
27	59	214 bp	198 bp	215 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]9	34
27'	59	214 bp	198 bp	215 bp	[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8	34
27"	59	214 bp	198 bp	215 bp	[TCTA]5 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]9	716
27.2		216 bp	200 bp	217 bp		SGM Plus
27.3		217 bp	201 bp	218 bp		SGM Plus
28	61	218 bp	202 bp	219 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10	34
28'		218 bp	202 bp	219 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]9	490
28.2	62	220 bp	204 bp	221 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10	716
28.2'		220 bp	204 bp	221 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8 TA TCTA	490
28.3		221 bp	205 bp	222 bp		SGM Plus
29	63	222 bp	206 bp	223 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	34
29'	63	222 bp	206 bp	223 bp	[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10	490
29.1		223 bp	207 bp	224 bp		SGM Plus
29.1		223 bp	207 bp	224 bp	[TCTA]4 [TCTG]6 [TCTA]6 TCA [TCTA]2 TCCA TA [TCTA]11	GenBank AP000433
29.2	64	224 bp	208 bp	225 bp	[TCTA]5 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10 TA TCTA	490
29.3		225 bp	209 bp	226 bp		SGM Plus
30	65	226 bp	210 bp	227 bp	[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	34
30'	65	226 bp	210 bp	227 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	490
30"	65	226 bp	210 bp	227 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	312
30"'	65	226 bp	210 bp	227 bp	[TCTA]6 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10	404
30.1		227 bp	211 bp	228 bp		SGM Plus
30.2	66	228 bp	212 bp	229 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10 TA TCTA	34
31	67	230 bp	214 bp	231 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	716
31'	67	230 bp	214 bp	231 bp	[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	34
31"	67	230 bp	214 bp	231 bp	[TCTA]6 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	490
31"'	67	230 bp	214 bp	231 bp	[TCTA]7 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	312
31.1		231 bp	215 bp	232 bp		SGM Plus
31.2	68	232 bp	216 bp	233 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11 TA TCTA	34
31.3		233 bp	217 bp	234 bp		SGM Plus
32	69	234 bp	218 bp	235 bp	[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13	34
32'	69	234 bp	218 bp	235 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13	490
32.1		235 bp	219 bp	236 bp		SGM Plus
32.2	70	236 bp	220 bp	237 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 TA TCTA	34
32.2'	70	236 bp	220 bp	237 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA	404
32.2"	70	236 bp	220 bp	237 bp	[TCTA]5 [TCTG]6 [TCTA]2 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA	404
32.3		237 bp	221 bp	238 bp		SGM Plus
33	71	238 bp	222 bp	239 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]14	490
33.2	72	240 bp	224 bp	241 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA	34
33.2'	72	240 bp	224 bp	241 bp	[TCTA]6 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13 TA TCTA	404
33.2"	72	240 bp	224 bp	241 bp	[TCTA]6 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12 TA TCTA	404
33.3		241 bp	225 bp	242 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]8 TCA [TCTA]2 TA TCTA	404
34	73	242 bp	226 bp	243 bp	[TCTA]10 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	404
34'	73	242 bp	226 bp	243 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]15	490
34.2	74	244 bp	228 bp	245 bp	[TCTA]4 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]14 TA TCTA	34
34.3		245 bp	229 bp	246 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]10 TCA [TCTA]4TA TCTA	404
35	75	246 bp	230 bp	247 bp	[TCTA]11 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	404
35'	75	246 bp	230 bp	247 bp	[TCTA]10 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	716
35.1		247 bp	231 bp	248 bp		*variant allele*
35.2	76	248 bp	232 bp	249 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]15 TA TCTA	490
35.3		249 bp	233 bp	250 bp		SGM Plus
36	77	250 bp	234 bp	251 bp	[TCTA]10 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13	404
36'	77	250 bp	234 bp	251 bp	[TCTA]11 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	716
36"	77	250 bp	234 bp	251 bp	[TCTA]10 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	404
36.2		252 bp	236 bp	253 bp	[TCTA]5 [TCTG]6 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]16 TA TCTA	490
36.3		253 bp	237 bp	254 bp		SGM Plus
37	78	254 bp	238 bp	255 bp	[TCTA]9 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	404
37'	78	254 bp	238 bp	255 bp	[TCTA]11 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13	716
37.2		256 bp	240 bp	257 bp		SGM Plus
38	79	258 bp	242 bp	259 bp	[TCTA]9 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13	404
38'	79	258 bp	242 bp	259 bp	[TCTA]13 [TCTG]5 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]12	716
38"	79	258 bp	242 bp	259 bp	[TCTA]10 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]13	404
38"'	79	258 bp	242 bp	259 bp	[TCTA]11 [TCTG]11 [TCTA]3 TA [TCTA]3 TCA [TCTA]2 TCCA TA [TCTA]11	404
38.2		260 bp	244 bp	261 bp		SGM Plus

CSF1PO

Other Names	Chromosomal Location	GenBank Accession
CSF	5q33.3-34; human c-fms proto-oncogene for CSF-1 receptor gene	X14720; has 12 repeats

Repeat: [AGAT] = GenBank top strand (commonly used)

Reported Primers	Ref.	PCR Primer Sequences
Set 1	3	5'-AACCTGAGTCTGCCAAGGACTAGC-3' (AGAT strand) 5'-TTCCACACACCACTGGCCATCTTC-3' (TCTA strand)
Set 2	Promega	GenePrint® PowerPlex™ 1.1
Set 3	ABI	AmpFlSTR® COfiler™

PCR Product Sizes of Observed Alleles

Allele (Repeat #)	Set 1 & Set 2	Set 3	Repeat Structure	Ref.
6	291 bp	281 bp	[AGAT]6
7	295 bp	285 bp	[AGAT]7	153
8	299 bp	289 bp	[AGAT]8	28
9	303 bp	293 bp	[AGAT]9	28
9.1	302 bp	294 bp		*variant allele*
10	307 bp	297 bp	[AGAT]10	28
10.1	308 bp	298 bp		*variant allele*
10.3	310 bp	300 bp		Budowle 1998
11	311 bp	301 bp	[AGAT]11	28
12	315 bp	305 bp	[AGAT]12	28
12.1	316 bp	306 bp		Budowle 1998
13	319 bp	309 bp	[AGAT]13	28
14	323 bp	313 bp	[AGAT]14	28
15	327 bp	317 bp	[AGAT]15	285
16	331bp	321 bp		Margolis-Nunno,H., et. al, 2001

Autor:

Osvaldo Castillo Ugarte

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

FACULTAD DE DERECHO

DIRECCIÓN DE INVESTIGACIÓN

TESIS DE INVESTIGACIÓN JURIDICA:

PERSPECTIVA MEDICO – LEGAL
PERSPECTIVA JURIDICA

Santiago, Diciembre de 2005

Partes: 1, 2, 3, 4, 5, 6, 7, 8, 9

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